Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Vector Laboratories Casein Solution 10X 250 ml
Casein Solution is a general blocking agent for immunohistochemistry assays, nucleic acid and protein blotting, and other applications. In this product, it is supplied as a 10x concentrate.
Teknova 1X PBS Solution, pH 7.4. 1000mL, Sterile
135.0mM Sodium Chloride, 2.7mM Potassium Chloride, 4.3mM Disodium Phosphate, 1.4mM Monopotassium Phosphate
Teknova 20X HBS-EP+ Buffer, pH 7.6.1000mL, Sterile.
3M Sodium Chloride, 200mM HEPES Free Acid,60mM EDTA, 1.0% Tween-20
Sigma Aldrich Fine Chemicals Biosciences Trizma base, 77-86-1, MFCD00004679, 100 g
Linear Formula: NH2C(CH2OH)3, Molecular Weight: 121.14, BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration), Synonym: 2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol.
Sigma Aldrich Fine Chemicals Biosciences Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Phosphate buffered saline BioPerformance Certified, pH 7.4 | MFCD00131855 | 10PAK
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.6, pH Adjusted with KOH) - 250ml
The HEPES [4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid)] Buffer is a versatile Good's zwitterionic buffering agent widely used in various applications in biochemistry and molecular biology. It maintains a stable pH in the range of 6.8 to 8.2, making it ideal for various applications at physiological pH, including cell culture, protein purification, isoelectric focusing, enzyme studies, bioprocessing, and live cell imaging. This buffer is adjusted with KOH.
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.5) - 1L
The Tris-HCl Buffer is a widely used bufering reagent in molecular biology and biochemistry, acting to maintain a stable pH, and preserve the integrity of target macromolecules. The buffering capacity of Tris-HCl is effective in the pH range of 7.0 to 9.0, making it ideal for many biological applications like cell culture, molecular biology, DNA/RNA extraction, electrophoresis, enzyme assays and immunohistostaining.
Sigma Aldrich Fine Chemicals Biosciences Phosphate Buffer Solution | 14265-44-2 | MFCD00145108 | 1 gal
Phosphate Buffer Solution | Mol Wt: 94.97 | 14265-44-2 | MFCD00145108 | 1 gal
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (pH 7.4, Sterile-Filtered) - 1L
Phosphate Buffered Saline (PBS) is a versatile buffer solution widely used in biological research due to its isotonic and non-toxic nature to cells. With a pH range of 7.0 to 7.4, PBS mimics the osmolarity and ion concentrations of the human body, making it ideal for a variety of applications including protein purification, as wash buffer in Western blot and immunoassays, sample dilution, and as a transport medium for tissue samples as well as RNA viruses.
Sigma Aldrich Fine Chemicals Biosciences Tris buffered saline with Tween(R) 20 BioUltra, tablet (for 500 mL), pH 7.6 | 10TAB
Tris buffered saline with Tween(R) 20 BioUltra, tablet (for 500 mL), pH 7.6 | 10TAB
Mitegen Tris/ HCl, 1M, pH8.5, 100 ml, CAS # 77-86-1
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Teknova 1M Tris-HCl, pH 8.0. 1000mL, Sterile, Endotoxin Tested, PCR Grade.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
Asuragen Inc TSMIII Storage Buffer, 50 mL, 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III, pH 7.0, -15 to -30°C
Asuragen TSMIII Storage Buffer is a specialized buffered solution designed to maintain stability for protein-coated nucleic acids. This reagent is ideal for the dilution of Asuragen's Armored products, ensuring the integrity of these sensitive biological materials through multiple freeze-thaw cycles. The stability provided by this storage buffer allows for flexible and scalable testing processes in various molecular assay applications.
- Volume: 50 mL
- Composition: 10 mM Tris, 100 mM NaCl, 1 mM MgCl2, 0.1% Gelatin, 0.3% Microcide III
- pH: 7.0
- Primary application: Dilution of Asuragen's Armored products
- Key benefit: Provides stability to protein-coated nucleic acids
- Storage stability: Withstands multiple freeze-thaw cycles
- Recommended storage temperature: -15 to -30 °C (secondary)
- Testing process benefit: Enables flexible and scalable testing processes
Zymo Research Corporation DNA Wash Buffer (Concentrate), 24 ml
This ensures high purity DNA to be recovered. Ethanol must be added to the concentrate prior to use. Instructions for each DNA Wash Buffer (concentrate) size is listed on the bottle and within the Zymo Research protocols it is used in.